RNA

Part:BBa_K4050019:Design

Designed by: Kelly Styles   Group: iGEM21_Victoria_Wellington   (2021-09-30)


20 nt spacer sgRNA for spermidine synthase gene in S. elongatus UTEX 2973


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Had to ensure off-target binding targets were minimal.


Source

Synechococcus elongatus UTEX 2973 spermidine synthase gene (speE - CP006471.1:2636619..2637479).

References